Jurnal Kesehatan Siliwangi

OPTIMASI VARIASI VOLTASE DAN WAKTU TERHADAP KUALITAS PITA DNA ESCHERICHIA COLI PADA PROSES ELEKTROFORESIS GEL AGAROSA

afifah nur amani putri — 2024-12-30

Background: Escherichia coli is one of the causes of foodborne illness. Conventional PCR is a PCR method that is carried out qualitatively followed by visualization on agar electrophoresis. Agarose gel electrophoresis is a technique that is often used in various fields of science to separate a mixture of DNA on agarose substrate. This method is used to perform qualitative analysis of DNA samples. In electrophoresis, there are factors that affect the movement of DNA molecules, one of which is voltage. In addition, the length of time of the electrophoresis process can also affect the effectiveness of the results and the rate of migration. When the electrophoresis process time is short, large DNA fragments still tend to stick. Purpose: Therefore, it is necessary to balance the voltage and time given to get good DNA banding results. Methods: The research unit that will be used is the result of amplification of Escherichia coli 16SrRNA gene DNA measuring 584 bp. The electrophoresis process was carried out with voltage variations of 50, 100, 150 volts and time variations of 30, 45, 60 minutes. Observation of electrophoresis results that have formed DNA bands will be measured the area of DNA bands using ImageJ application. Conclusion: Based on the results of the study concluded that: The optimum voltage in the electrophoresis process in obtaining good E.coli DNA bands is at a voltage of 150 volts. The optimum time in the electrophoresis process in obtaining good E.coli DNA bands is for 30 minutes.

OPTIMASI KONSENTRASI DAN WAKTU KONTAK EKSTRAK DAUN JATI (Tenctona grandis) SEBAGAI ALTERNATIF SAFRANIN PADA PEWARNAAN GRAM

Andita Izmi Syafitri — 2024-12-30

Safranin is a cationic dye and is one of the harmful substance, in addition to its relatively expensive price, safranin waste also has several harmful effects on health, so the various studies on the use of natural dyes as an alternative of safranin have been widely carried out. Teak leaves are one of the plants that have the potential to used as an alternative of safranin because they contain anthocyanin compounds. This study aims to determine the concentration and optimal contact time of teak leaf extract that can be used as an alternative of safranin. The research method used is a quasi-experiment by providing treatment with 25%, 50%, and 100% concentration variations also contact time variations of 0.5, 1, and 1.5 minutes. The results showed that teak leaf (Tectona grandis) extract could be used as an alternative of safranin in Gram staining of E. coli and S. aureus. The conclusion of this study is that the optimum concentration of teak leaf extract is 50%, and the optimum contact time of teak leaf extract in Gram staining is 0.5 minutes.

PENGARUH PENYIMPANAN ARSIP BLOK PARAFIN TERHADAP KUALITAS PREPARAT JARINGAN

Dinda Nurdianti — 2024-12-30

Paraffin block archive storage is the process of maintaining and managing used paraffin blocks. "A standardized paraffin block archive storage system will facilitate access for diagnostic purposes and help maintain the quality of the paraffin blocks over the long term. If the storage system is not standardized, it can cause several problems, such as delaying diagnostic services and reducing the quality of tissue preparations. Most paraffin blocks can be stored at temperatures of 20-27°C. Paraffin blocks should not be exposed to temperatures exceeding 27°C, as improper storage can cause the paraffin to soften and damage the tissue, reducing the quality of tissue preparations. This study aims to determine the effect of paraffin block archive storage on tissue preparation quality. The research samples used paraffin block archives stored in cabinets for 5 and 6 years, and paraffin block archives stored in plastic for 5 and 6 years. The quality of tissue preparations was assessed based on the clarity of the arrangement of bile duct cell walls, namely mucosa, lamina propria, muscularis and serosa microscopically and the color contrast of nuclei and cytoplasm using ImageJ software. The results of the study showed that there was no significant effect of paraffin block archive storage on tissue preparation quality. Further research needs to be conducted on the storage duration of paraffin block archives in wooden and plastic cabinets over a period of 10 years, as well as obtaining information related to the initial condition of the paraffin blocks, including color, texture, shape,and tissue authenticity.

EFEKTIVITAS SUHU DAN WAKTU PENYIMPANAN AIR MINUM ISI ULANG TERHADAP HASIL MOST PROBABLE NUMBER (MPN) COLIFORM

Farah Zahra Amalia — 2024-12-30

Refillable drinking water depots have currently become the most widely chosen source of water by the community due to their relatively affordable prices and easy accessibility around residential areas. However, the selection of these refillable drinking water depots can pose risks that can endanger health. Although they have undergone strict processing and packaging, there is a possibility that refillable drinking water can be contaminated by Coliform. One of the factors that can affect the level of Coliform contamination in refillable drinking water is the storage temperature and time.This study aims to determine the effectiveness of storage temperature and time of refillable drinking water on the results of the Most Probable Number (MPN) Coliform test. The type of research used is Quasi-Experiment. Refillable drinking water samples were taken from 30 houses around SMAN 1 Kota Sukabumi. The samples were divided into 2 storage temperature treatments (25°-30°C and 70°-80°C) with storage times of 3 days. The results showed that there was a significant influence between temperature and storage time treatments on the MPN Coliform results of refillable drinking water. The 70°-80°C temperature treatment with 1, 2, and 3 days of storage time resulted in the lowest MPN Coliform, while the 25°-30°C treatment with an average of 27.10°C and 3 days of storage time resulted in the highest MPN Coliform. It can be concluded that the storage of refillable drinking water at a temperature of 27.10°C for 3 days is the most effective treatment in determining the MPN Coliform.

PENGARUH PENINGKATAN KADAR TRIGLISERIDA PADA SERUM LIPEMIK TERHADAP KADAR ASAM URAT METODE URICASE

fatthiyyahkaamilaridwansyah ridwansyah — 2024-12-30

The quality of the serum can be affected by several factors such as hemolyzed, icteric, and lipemic serum. These factors might cause inaccurate test results, for example that lipemic serum can increase uric acid level. This study aims to determine whether or not there is an effect of triglyceride level in lipemic serum and to determine how much triglyceride level can affect uric acid level using the uricase method. This study was conducted in May 2024 at the Clinical Chemistry Laboratory of Medical Laboratory Technology, Poltekkes Bandung. The research method is a laboratory experiment with a statistical group comparison research design. The sample in this study was serum from 6 students of Medical Laboratory Technology, Poltekkes Kemenkes Bandung with the criteria of not hemolyzed, not icteric, and not lipemic. The serum was made lipemic by adding egg yolk so that the triglyceride level varied between 461 to 2492 mg/dL. In the lipemic serum, blood uric acid level were examined using the uricase method. The results showed that uric acid level in lipemic serum increased along with increasing triglyceride level. The results of the General Linear Model (GLM) Statistical Test showed that lipemic serum with triglyceride level of 1028 mg/dL had an effect, while lipemic serum with triglyceride level of 461 to 546 mg/dL did not affect uric acid level using the uricase method. The conclusion of this study is that triglyceride levels starting from 1028 mg/dL have an influence on increasing uric acid levels using the uricase method by 97,08%.

PERBANDINGAN METODE KATO – KATZ DENGAN METODE STOLL UNTUK PEMERIKSAAN TELUR CACING SOIL TRANSMITTED HELMINTH PADA SAMPEL FESES

Hanayori Octania nugraha — 2024-12-30

Soil transmitted helminth (STH) is a group of intestinal parasitic worms of the nematode class that can cause human infection through soil contaminated with their eggs or larvae. Soil transmitted helminth types that most commonly infect humans are Ascaris lumbricoides (A. lumbricoides), Trichuris trichiura (T. trichiura) and hookworms (Necator americanus (N. americanus) and Ancylostoma duodenale (A. duodenale). The intended purpose of this study is to determine the similarity of worm egg identification results between the Kato-Katz and the Stoll method. The statistical analysis applied in this study is Paired T test. The results of this study showed that the Kato-Katz and Stoll methods applied to five feces samples indicated Soil Transmitted Helminth (STH) worms in the laboratory of the Poltekkes Kemenkes Bandung found the presence of the two most common types of worm eggs; Ascaris lumbricoides and Trichuris trichiura. Meanwhile, hookworm eggs were not found in the two methods. It was found that the number of Ascaris lumbricoides eggs was more when compared to Trichuris trichiura. Statistical data identified that both methods had a normal distribution (Shapiro-Wilk test) with a difference (Paired T_test, p=0.024). Where the most eggs were found in the Kato-Katz method. The Kato-Katz and Stoll methods for Trichuris trichiura had a correlation (r=0.893, p=0.0001). The sensitivity of these two methods is 100%, but the specificity cannot be calculated because all samples were positive for STH. Therefore, WHO recommends the Kato-Katz method in the examination of STH infection because it is superior in the detection of worm eggs.

POTENSI EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb) DALAM MENGHAMBAT PERTUMBUHAN Streptococcus pyogenes

Lia Nurdini — 2024-12-30

S. pyogenes is the primary cause of acute pharyngitis. The inappropriate, repeated, and irregular use of antibiotics can lead to resistance. One alternative is to utilize plants containing antibacterial compounds. This study aims to identify the concentration and contact time of fragrant pandan leaf extract that has the potential to inhibit the growth of S. pyogenes. The method applied in this study is the disk diffusion test with fragrant pandan leaf extract at concentrations of 20%, 40%, and 60%, with contact periods of 24 hours and 48 hours. Bacitracin was used as a positive control, and 10% DMSO as a negative control. The data were analyzed using a Two Way ANOVA test, showing a significant difference between the extract concentration and contact time on the inhibition zone diameter (p<0.05). The study resulted in an average inhibition zone diameter at a 20% concentration of 9 mm after 24 hours, 40% at 9.9 mm, and 60% at 11 mm. For the 48-hour contact time, the average inhibition zone diameter at a 20% concentration was 8.9 mm, 40% was 9.7 mm, and 60% was 10.8 mm. The average inhibition zone diameter in the positive control (bacitracin) was 18.3 mm, while the negative control showed no inhibition zone.

PERBANDINGAN PEMERIKSAAN TINJA ANTARA METODE FLOTASI DAN METODE RITCHIE DALAM MENDETEKSI SOIL TRANSMITTED HELMINTH

Nayla Wasilah — 2024-12-30

Microscopic stool examination has two examinations, namely qualitative and quantitative examination. Methods that can be done for qualitative fecal examination are direct slide method, anal swab method, flotation method, tape method, and sedimentation method. While in quantitative examination, namely Stoll, Ritchie, and Kato Katz. This study aims to determine the difference in the identification results of the number of eggs of each species found in the Flotation Method and the Ritchie Method. This research was conducted at the Parasitology Laboratory of the Polytechnic of the Ministry of Health Bandung in March-April 2024. The population in this study was obtained from feces suspected of Soil Transmitted Helminth. The number of samples used in this study were 30 samples using purposive sampling method, namely sampling techniques with certain criteria. Data analysis used the Paired Sample T Test statistical test by calculating the sensitivity and specificity values. The results showed that the number of Soil Transmitted Helminth eggs was found more in the ritchie method, namely in Ascaris lumbricoides 350 (84.95%) and Trichuris trichiura 62 (15.05%), while in the flotation method in Ascaris lumbricoides 195 (85.15%) and Trichuris trichiura 34 (14.85%). Flotation method and Ritchie method have the same sensitivity and specificity values, namely 100% sensitivity and 0% specificity. The results of the Paired Sample T Test have a significant value of 0.001 (p < 0.05) indicating that there is a difference in the results of STH examination with flotation and ritchie methods.

PERBEDAAN SUHU DAN WAKTU PENYIMPANAN SPESIMEN SPUTUM TERHADAP HASIL PEMERIKSAAN MIKROSKOPIS BAKTERI TAHAN ASAM

Nianda Alfi Agilia Saputri — 2024-12-30

Sputum specimens should ideally be examined immediately to ensure accurate BTA (Acid Resistant Bacteria) microscopic examination results. The limited number of ATLMs while the large amount of sputum causes delays in examination. The purpose of the study was to determine the differences in characteristics and the number of BTA based on the International Union Againtst Tuberculosis and Lung Disease (IUATLD) scale of sputum examined immediately with those stored at temperatures (23-27°C) and (35-39°C) with storage times of 6.5 and 7.5 hours. The research design used was quasi-experimental. The results showed there were changes in the characteristics of sputum stored at temperatures (23-27°C) and (35-39°C) with storage times of 6.5 and 7.5 hours against sputum examined immediately. Sputum examined immediately had a BTA count of 1+. Sputum stored at temperature (23-27°C) at storage times of 6.5 and 7.5 hours the number of BTA is 1+, while sputum stored at temperature (35-39°C) at storage times of 6.5 and 7.5 hours the number of BTA is 2+. So it can be concluded that there are changes in the characteristics of sputum stored at temperatures (23-27°C) and (35-39°C) with storage times of 6.5 and 7.5 hours. There is no difference in the number of BTA scale (IUATLD) of sputum stored at temperature (23-27°C) at storage times of 6.5 and 7.5 hours, while sputum stored at temperature (35-39°C) at storage times of 6.5 and 7.5 hours there is a difference in the number of BTA scale (IUATLD) of sputum specimens examined immediately.

PERBEDAAN KADAR KREATININ METODE JAFFE REACTION PADA SERUM SEGERA DAN SIMPAN SELAMA 1, 2, DAN 3 HARI PADA SUHU RUANG

Niva Salsabila Widayat — 2024-12-30

The laboratory examination stage consists of pre-analytical, analytical and post-analytical stages. One of the laboratory examination stages is the pre-analytical stage, where this stage has the largest error percentage, namely 77.1%. Checking creatinine levels is one of the tests to determine kidney function. This study aims to determine differences in serum creatinine levels immediately and stored for 1, 2 and 3 days at room temperature. This research was conducted in April 2024 at the Clinical Chemistry Laboratory, Technology Medical Laboratory, Health Polytechnic, Ministry of Health, Bandung. This research was quasi-experimental in nature by providing treatment in the form of storing serum after centrifugation for 1, 2, and 3 days at room temperature and then the levels were compared with the serum levels which were immediately examined. This examination was examined using the Jaffe reaction method using a photometer with a wave length of 505 nm and repeated in triplicate so that 72 data were obtained which were then processed using SPSS with the general linear model (GLM) test. The results of the study showed that the creatinine examination showed an average immediate serum value of 0.88 mg/dL and stored serum for 1 day 0.85 mg/dL, 2 days 0.81 mg/dL, 3 days 0.76 mg/dL (P < 0.05) which means there is a difference in the results of the immediate serum creatinine examination and the stored serum.

OPTIMASI MEDIA ALTERNATIF NUTRIENT AGAR BERBAHAN KACANG MERAH (Phaseolus vulgaris L.) DALAM MENUMBUHKAN Staphylococcus aureus

Nuri Mutiara Sulam — 2024-12-30

Media are used to grow bacteria to diagnose infectious diseases, stock bacterial cultures, and in educational institutes for learning and research on the characteristic of bacteria. One of these media is nutrient agar which contains nutrients in the form of protein and carbohydrates. Growth media has less affordable prices so alternative media are created. Alternative media to replace nutrient agar with a vegetable protein source to replace the protein contained in the media. For example, the vegetable protein used is from red beans. Red beans contain protein and amino acids which will replace protein in nutrient agar media. This research aims to determine alternative media made from red beans that can be used as a substitute for nutrient agar, the optimum concentration and boiling time of red beans in growing Staphylococcus aureus with concentration variations of 5%, 10%, and 15% and time variations of 5 minutes, 10 minutes, and 15 minutes. In the research, inoculation was carried out using the pouring cup method. The results of the number of colonies on the alternative media will be compared with the results of the number of colonies on the control, namely nutrient agar. Based on the Generalized Linear Model (GLM) statistical test, an alternative media with a concentration of 10% and a boiling time of 10 minutes can be used as a substitute for nutrient agar.

PERBANDINGAN LAMA WAKTU PENUNDAAN SPESIMEN FESES TERHADAP PEMERIKSAAN SOIL TRANSMITTED HELMINTH DENGAN METODE KATO−KATZ

Putri Jenita Nurrafikasari — 2024-12-30

Soil transmitted helminth (STH) is a parasitic worm whose spread requires soil media. The spesieses of STH that infect humans are Ascaris lumbricoides, Trichuris trichiura, Necator americanus, and Ancylostoma duodenale. The Kato-katz method is easy to perform but requires accuracy in counting the number of worm eggs. The number of worm eggs found can be influenced by the length of time the sample is delayed. This research aims to determine the difference in the number of STH eggs based on the length of time delayed fresh feces samples in less than 3 hours and delayed feces samples less than 72 hours using the Kato-katz method. The research was conducted with feces samples from Padjadjaran University which indicated the presence of STH eggs. The results found in the delayed feces samples < 72 hours eggs are less than in fresh feces samples in < 3 hours. The number of Ascaris lumbricoides eggs in fresh samples in < 3 hours was 4,950 EPG while in samples delayed < 72 hours was 3,495 EPG. The number of Trichuris trichiura eggs in fresh samples in < 3 hours was 510 EPG while in samples delayed < 72 hours was 450 EPG. Based on Twoway Anova test obtained sig value. < 0.05 from the time delay to the results of the eggs found, namely 0.037. So, it can be interpreted that there is an influence in the length of time delayed examination of feces samples on the number of Soil transmitted helminth eggs found.

OPTIMASI WAKTU INKUBASI VAKSIN H5N1 PADA TELUR AYAM BEREMBRIO DENGAN SUHU 37OC TERHADAP TITER HEMAGLUTINASI

Rizka Fuji Lestary — 2024-12-30

Avian Influenza virus subtype H5N1 can infect animals as well as humans due to its high pathogenicity. Avian Influenza virus contains hemagglutinin protein that can agglutinate red blood cells. The purpose of this study was to determine the optimal incubation time, namely 3 days, 4 days, and 5 days and whether there are differences in the different incubation times. The type of research used was descriptive. The samples used were Avian Influenza Vaccine subtype H5N1 inoculated into embryonated chicken eggs aged 9-11 days. The allantois fluid produced from vaccine inoculation in embryonated chicken eggs can be tested for hemagglutination to determine its titer. The results showed that the optimum incubation time was 3 days and the optimum hemagglutination titer at the 3rd day incubation time was 1/256, at the 4th day incubation time was 1/256, and at the 5th day incubation time was 1/512. The data were analyzed using One-Way Anova test on SPSS. In conclusion, there was a significant difference in hemagglutination titer in the H5N1 vaccine inoculated in embryonated chicken eggs for 3 days and 4 days against the incubation time for 5 days.

STABILITAS REAKSI ENZIMATIK KADAR TRIGLISERIDA METODE GPO-PAP DENGAN VARIASI WAKTU INKUBASI

Salmanda Endrique Putri — 2024-12-30

One of the triglycerides test lipid profile examination that is often analyzed because it provides information about cardiovascular health and stroke. Laboratory services have an important meaning in making a diagnosis based on the patient's health history and are an integral part in achieving quality laboratory results that have accurate accuracy and precision. Analytical factors that can influence examination results include incubation time. The delay in the length of incubation time is due to the limited number of laboratory personnel and inadequate equipment. The objective of this study was to assess the impact of stability on serum triglyceride levels using the GPO-PAP method with different incubation durations. The research employed a quasi-experimental design, utilizing serum samples from three Medical Laboratory Technology students. The samples were subjected to varying incubation times of 10, 60, 75, 90, and 105 minutes at room temperature, with each condition being repeated five times. The mean results of serum triglyceride levels with incubation times of 10, 60, 75, 90, and 105 minutes respectively were 80,0 mg/dL, 79,00 mg/dL, 76.36 mg/dL, 73.65 mg/ dL, and 67.94 mg/dL. Data analysis was conducted using the General Linear Model (GLM) test, with significance indicated by the Sig value. < 0.05 means that triglyceride levels with an incubation time of 75 minutes are statistically unstable.

STABILITAS WAKTU PENYIMPANAN SERUM, PLASMA K3EDTA, DAN PLASMA HEPARIN PADA SUHU RUANG TERHADAP PEMERIKSAAN TRIGLISERIDA

Salsabilla Naqiyyah — 2024-12-30

Triglyceride examination in the laboratory sometimes experiences delays caused by several factors, such as damage to the equipment, running out of reagents, and obstacles when sending reference samples. Stability is the ability of a sample to maintain the initial value of the measured analyte within a certain period under specified storage conditions. One of the factors that can affect stability is storage temperature. The purpose of this study was to determine the stability of triglyceride levels in serum, K3EDTA plasma, and heparin plasma which were examined immediately and stored at room temperature for 1, 2, and 3 days. This type of research is experimental research with a quasi-experimental research design, which provides storage treatment for serum specimens, K3EDTA plasma, and heparin plasma after centrifugation. The statistical test used was the General Linear Model (GLM) test. The results showed that serum and heparin plasma specimens had significant differences (Sig.0.000) in 2-day storage. While the K3EDTA plasma sample had significant differences (Sig.0.009) in 3-day storage. So it can be concluded that there is a difference in the stability of triglyceride levels in serum, K3EDTA plasma, and heparin plasma after storage at room temperature. Triglyceride levels in serum and heparin plasma specimens are stable for 1 day at room temperature. While triglyceride levels in K3EDTA plasma are stable for 2 days at room temperature. However, there is no clinical difference in serum, K3EDTA plasma, and heparin plasma specimens.

EFEKTIVITAS KONSENTRASI DAN WAKTU MASERASI EKSTRAK DAUN BELUNTAS (PLUCHEA INDICA L.) TERHADAP PERTUMBUHAN STREPTOCOCCUS PYOGENES

Siti Ismi Yulandari — 2024-12-30

One of the most common diseases in Indonesia, namely pharyngitis, is caused by Streptococcus pyogenes. Inappropriate treatment of pharyngitis with antibiotics can result in bacteria that are resistant to antibacterials. Therefore, herbal plants that are effective as natural antibacterials are needed, such as beluntas leaves (Pluchea indica L.) to prevent this. Apart from that, the right method is needed so that the contents of the beluntas leaves can be attracted properly. The aim of this research was to determine the effective concentration and maceration time of beluntas leaf extract in inhibiting the growth of S.pyogenes. This research used varying maceration times of 24 and 72 hours. Then, from each variation of maceration time, beluntas leaf extract was made in concentrations of 5%, 10%, 15%, 20%, and 25%. Beluntas leaf extract was tested for its inhibitory power against S.pyogenes using the Kirby Bauer method. The data obtained was the diameter of the inhibitory power of beluntas leaf extract on the growth of S.pyogenes, then the data was processed statistically using the Kruskal-Wallis test and a further test, namely the Post Hoc Test. The results of this research were that beluntas leaf extract which was macerated for 72 hours with a concentration of 20% and 25% had an average diameter of inhibition against S.pyogenes of 9.04 mm and 12.71 mm. Therefore, a maceration time of 72 hours with a concentration of 25% is effective in inhibiting the growth of S.pyogenes.

PERBANDINGAN KUALITAS WARNA PADA PREPARAT JARINGAN MENGGUNAKAN METIL ESTER SULFONAT DAN XYLOL SEBAGAI AGEN DEPARAFINISASI

Syafa Aulia Rahmah — 2024-12-30

Deparaffinization is the process of decaying the remnants of paraffin in tissue preparations. Deparaffinization aims to make the dye that will be given to the preparation fully absorbed so that the preparation can be colored properly. This deparaffinization process uses xylol solution in its immersion. Methyl Ester Sulfonate (MES) is one of the vegetable oil-based anionic surfactants that can dissolve in water and has a long carbon chain that is soluble in oil and vaseline. The purpose of this study was to determine whether Methyl Ester Sulfonate can be used as a deparaffinizing agent for tissue preparations. The sample in this study was appendiceal tissue as many as 30 units, each unit of tissue made into 2 tissue preparations so that it became 60 tissue preparations, namely 30 tissues using xylol as a deparaffinizing agent and 30 tissues using methyl ester sulfonate. The parameters of this study were the clarity of the color of the nucleus and cytoplasm, the uniformity of cell color, and the contrast of the color of the nucleus and cytoplasm qualitatively and quantitatively. The statistical test used was Independent T-Test test if the data were normally distributed and Mann Whitney test if the data were not normally distributed. Based on the Independent T-Test test, the Sig value was obtained. 0.951 and 0.102, both of which are >0.05, meaning that there is no significant difference between the use of xylol and 10% methyl ester sulfonate as a deparaffinizing agent.

PERBEDAAN KADAR BILIRUBIN TOTAL SERUM LIPEMIK SEBELUM DAN SESUDAH PENAMBAHAN POLYETHYLENE GLYCOL

Syifa Alifah Nuraini — 2024-12-30

Lipemic serum is characterized by its turbid appearance, resulting from elevated concentrations of lipoproteins, particularly chylomicrons and very low-density lipoproteins (VLDL). This turbidity can interfere with the accuracy of total bilirubin measurements, leading to potentially inaccurate results. To mitigate this issue, polyethylene glycol (PEG) is often used as a clarifying agent. This study aims to evaluate the differences in total bilirubin levels in lipemic serum before and after
the addition of 10% polyethylene glycol 6000. This quasi-experimental research uses a one group pretest-posttest design. conducted in May 2024 at the Clinical Chemistry Laboratory, Department of Medical Laboratory Technology, Bandung Health Polytechnic. A total of 30 serum samples from hospitals in Bandung were combined to make pooled sera, then modified to be lipemic using egg yolk until the triglyceride level reached 991.5; 1002.8; and 1067.8 mg/dL.Total bilirubin levels
were measured both before and after the addition of polyethylene glycol, with initial levels recorded at 1.62, 2.64, and 3.58 mg/dL, and post-PEG levels at 2.40, 3.30, and 4.35 mg/dL. Statistical analysis using a paired sample t-test revealed a significance level of 0.000 (p < 0.05), indicating a significant difference in total bilirubin levels before and after the application of polyethylene glycol in lipemic serum.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN CENGKEH (Syzygium aromaticum L.) TERHADAP Streptococcus pyogenes

Syifa Marha Ghaisani — 2024-12-30

One of the most common bacterial infections is pharyngitis. Most cases of acute pharyngitis are caused by viruses, but the main bacterial agent is Streptococcus pyogenes. Some antibiotics have been resistant to Streptococcus pyogenes, so alternative antibacterials are needed, namely the use of clove leaves (Syzygium aromaticum L.). The type of research used is Quasi Experiment. This study was conducted to test the antibacterial activity of clove leaves against Streptococcus pyogenes. Clove leaf extract was obtained by maceration method using 96% ethanol solvent. Phytochemical tests on clove leaf extracts showed positive leaves containing saponins, phenols, terpenoids, flavonoids, and tannins. Antibacterial activity test was conducted by disc diffusion method with variation of extract concentration of 10%, 15%, and 25% and incubation time for 24 and 48 hours. Bacitracin disc was used as positive control and negative control used 10% DMSO. The test results showed that there was antibacterial activity indicated by the formation of inhibition zone at 25% concentration with an average of 9.23 mm and 15% concentration of 8.8 mm. The zone of inhibition formed did not match the zone of inhibition in the control (≥13 mm).

FAKTOR-FAKTOR YANG BERHUBUNGAN DENGAN KEJADIAN HIPERTENSI PADA PASIEN DIABETES MELITUS TIPE 2 DI PUSKESMAS CIGUGUR TENGAH

Vika Ayu Lestari — 2024-12-30

Individuals with type 2 diabetes mellitus have elevated blood glucose levels (hyperglycemia). This results in an increase in body fluid volume and damages the vascular system and will cause peripheral arterial resistance to increase which ultimately causes hypertension. The prevalence of type 2 diabetes mellitus and hypertension in the Cigugur Tengah Health Center area is still increasing every year. The purpose of this study was to determine the factors associated with the incidence of hypertension in patients with type 2 diabetes mellitus at the Cigugur Tengah Health Center. This type of research is descriptive analytic research with a cross sectional research design. The population and sample of this study were type 2 diabetes mellitus patients with a doctor's diagnosis who were willing to become respondents and were registered in the Central Cigugur Health Center area, totaling 59 people. Sampling was carried out using the total population technique where the sample was treated the same as the population and using a questionnaire sheet. Data analysis using the Chi-Square Test using the SPSS program. The results showed that there was a significant relationship between the age factor with a value of P= 0.023 <0.05, the gender factor with a value of P= 0.037 <0.05, the drug consumption compliance factor with a value of P= 0.010 <0.05, the length of suffering with a value of P= 0.043 <0.05, and there was no relationship between the Body Mass Index factor with a value of P= 0.289>0.05 with the incidence of the disease.

PERBANDINGAN HASIL PEWARNAAN PREPARAT HISTOLOGI MENGGUNAKAN XYLOL DAN MINYAK JAGUNG YANG DIPANASKAN PADA PROSES DEPARAFINISASI

Zahra Mufidah — 2024-12-30

Deparaffinization removes residual paraffin from tissue, enabling optimal dye absorption in histological preparations. While xylol is commonly used, its high exposure risks necessitate exploring safer alternatives. The research compares the efficacy of corn oil 60°C to xylol as a deparaffinization agent. Heated corn oil is necessary to reduce viscosity and increase solubility. Corn oil can also be used as an environmentally friendly and cost-effective alternative. The research employed a descriptive approach, comparing staining results of kidney tissue preparations from control and experimental groups. Assessment criteria included color uniformity and contrast, determined by the difference in Optical Density values between nuclear and cytoplasmic staining. Due to non-normal data distribution and the comparison of two unpaired means, the Mann-Whitney U test was utilized for statistical analysis. The results obtained an Asymp sig value of 0.002 where the value is included <0.05 which explains that there is a significant different on the staining results of tissue preparations deparaffinized using xylol and corn oil 60°C with the percentage of staining results in the good category deparaffinized using corn oil only reached 26.67%. The results showed that there were differences in the staining results of kidney tissue preparations deparaffinized using xylol and 60°C corn oil so that 60°C corn oil has less effective to be an alternative deparaffinization agent to replace xylol.

EVIDENCE BASED CASE REPORT : PENGARUH EFFLEURAGE MASSAGE TERHADAP PENURUNAN NYERI PADA IBU BERSALIN KALA I

Aliya Ramadiyanti — 2024-12-30

Background : Labor pain can cause stress or fear which can result in increased secretion of catecholamine and cortisol levels, resulting in decreased uterine blood flow and decreased uterine contractility which can affect the labor process. One of the non-pharmacological actions that can be taken to reduce labor comfort is by using a massage or stroking method on the stomach, namely effleurage massage. Effleurage massage is a light massage using the fingers, usually on the stomach, in rhythm with breathing during contractions. Purpose : To applied the effleurage massage on reducing labor pain in the first stage. Method : The method used is to search for evidence through databases from Google Scholar and the Garuda Portal published in 2016-2023. Results : There was a decrease in labor pain scores using the Numeric Rating Scale (NRS) after effleurage massage. Conclusions : The application of effleurage massage can be applied to maternal in the first stage of labor to reduce the intensity of labor pain.

EBCR: PENGARUH KOMPRES AIR HANGAT TERHADAP PENGURANGAN NYERI PERSALINAN KALA I FASE AKTIF

Anastasya Deby Lestari — 2024-12-30

Labor pain is a common complaint experienced by women during childbirth, especially during the active phase of the first stage of labor. Previous research has shown that primiparous women tend to experience more intense pain compared to multiparous women. Objective: This study aims to evaluate the effectiveness of warm water compresses in reducing the intensity of labor pain during the active phase of the first stage of labor in women giving birth at UPTD Puskesmas Garuda. Methods: The study design used a case study of women in the active phase of the first stage of labor. The variables observed were the intensity of pain before and after the application of warm water compresses. Results: The results of the case study showed a significant decrease in pain intensity after the application of warm water compresses. Conclusion: Warm water compresses were proven to be effective in reducing the intensity of labor pain during the active phase of the first stage of labor in women giving birth at UPTD Puskesmas Garuda. Implications: These findings can be a clinical recommendation for healthcare workers, especially midwives, to use warm water compresses as one of the non-pharmacological methods for managing labor pain.

EVIDENCE BASED CASE REPORT (EBCR): PENGARUH HIPNOBIRTHING TERHADAP PENURUNAN NYERI PERSALINAN KALA 1 FASE AKTIF

Chagya Fadhilah — 2024-12-30

Background: Labor pain is a physiological process that occurs during labour, where individual responses to pain vary. So it requires efforts to overcome labor pain using pharmacological and non-pharmacological methods, one of which is the hypnobirthing method. Purpose: This application aims to provide a positive experience for mothers during labour with a sense of comfort and calm during the labour process. Method: The literature e-database PubMed, Cochrane, Science Direct and Google Scholar was used to search for evidence. Results: In this case, the results showed changes in labour pain using VAS measurements. Discussion: Hypnobirthing works by activating nerves in the brain, causing the release of natural morphine-like substances called enkephalins and endorphins. So that it can increase the mother's tolerance to pain. Conclusion: Hypnobirthing method affect reducing labour pain.

PENGARUH MUROTTAL AL-QURAN TERHADAP PENURUNAN TINGKAT KECEMASAN IBU BERSALIN

Dini Farhani — 2024-12-30

Background : Labor pain is one of the most intense pains a woman will experience in childbirth. The pain felt by the delivery mother will have an anxiety effect on the mother. Based on the results of the study, the highest level of anxiety felt by birth mothers out of 50 respondents. 17 of them (34%) experienced moderate levels of anxiety, 13 people with normal levels of anxiety, 15 people with mild levels of anxiety and 5 people with severe levels of anxiety. Therefore, non-pharmacological therapy is needed to reduce anxiety in birth mothers, one of which is by using a distortion technique, namely listening to the Quran’s murottal. Method : This journal search method uses keywords, Boolean operators and E-data Based according to predetermined criteria. Purpose: to determine the influence of murottal Al-Quran on anxiety of mothers giving birth. Results: After being given the intervention, the mother's anxiety score decreased from 89 to 40 after listening to Murottal for 1.5 hours. Conclusion: There is an influence on the anxiety of mothers giving birth by being given Al-Quran murottal for 1.5 hours, namely reducing the level of anxiety of mothers giving birth.

EFEKTIVITAS AROMATERAPI LAVENDER TERHADAP INTENSITAS NYERI PERINEUM PADA IBU POSTPARTUM : EVIDENCE BASED CASE REPORT

Fikriya Khoerani — 2024-12-30

Background: Perineal pain is physiological for mothers who have just given birth, but this pain interferes with their ability to move, which can cause complications such as postpartum bleeding. One non-pharmacological method that can be used is lavender aromatherapy to reduce the perineal pain you feel. Aromatherapy stimulates the hypothalamus, or pituitary gland, to release endorphins, which cause feelings of calm, serenity, and happiness. In addition, the active ingredients lavender, linaool and linalyl acetate, function as an analgesic. Purpose:The aim of this case study is to apply lavender aromatherapy to reduce perineal pain in post partum mothers. Method: The method used is searching for evidence through databases from PubMed and Google Scholar with publication years 2019 – 2023, full text, experimental research design/systematic review, in English or Indonesian. Results: The results of the case report show that lavender aromatherapy reduces perineal pain in post partum mothers who experience perineal wound pain, before being given the intervention they get a pain score of 6 and after being given the intervention for 1 hour they get a pain score of 3. Conclusion: lavender aromatherapy reduces perineal pain in mothers postpartum.

the EFEKTIVITAS BIRTHBALL TERHADAP NYERI PERSALINAN KALA I FASE AKTIF

Gianti Asni Fauziyah — 2024-12-30

Labor pain during the first stage is severe pain that is felt by the mother in labor for a longer time. Based on the Indonesian Hospital Association Data Center, it was explained that 16% of mothers in Indonesia experienced birth complications and 21% stated that the labor they experienced was a painful labor because they felt excruciating pain, while 63% did not receive information about labor complications. Several types of management for dealing with pain using non-pharmacological methods, which are preferred by birth mothers to deal with pain, are the distraction method with a birth ball. The method used is a case study of mothers giving birth. The aim of this case study is that students are able to apply and find out the effectiveness of birth balls as pain relief for mothers giving birth during the first active phase. The results of the case study showed that the birth ball was an effective way to reduce pain in the first stage of the active phase of labor.

EVIDENCE BASED CASE REPORT (EBCR) : PENGARUH PEMBERIAN PIJAT OKETANI TERHADAP PRODUKSI ASI

Jasmine Khoirul Barriyah — 2024-12-30

Background: Breastfeeding is a nutritional need for babies during growth and development. Breast milk (ASI) helps in sensory, cognitive, and motor development and protects and prevents babies from various diseases and infections. Breast milk production is influenced by the hormones prolactin and oxytocin, food intake, the mother's psychological condition, breast care, and the frequency of breastfeeding. In stimulating the release of these hormones, one of the efforts made is breast care. Breast care will stimulate the breasts and affect the hypopise to release more progesterone, estrogen, and oxytocin hormones. The hormone oxytocin will contract other cells around the alveoli, causing milk to flow down towards the nipple. One of the breast care techniques is oketani massage. This massage is a massage technique popularized in Japan that will make the breasts softer, the areola and nipples elastic, making it easier for babies to suckle breast milk. Objective: To determine the effect of oketani massage on postpartum maternal breast milk production. Method: This journal search uses keywords, Boolean Operators and E-data based with literature review and experimental research types. Results: Based on this study, it was found that there was an effect of Oketani massage on breast milk production in postpartum mothers. Conclusion: It is expected that health workers can use the Oketani massage technique to help breast milk production in postpartum mothers as an easy and inexpensive alternative that can help mothers in breast milk production.

The EVIDENCE BASED CASE REPORT (EBCR): PENGARUH PIJAT WOOLWICH TERHADAP KELANCARAN PRODUKSI ASI PADA IBU NIFAS

Jen Astika Nuari — 2024-12-30

Background: Exclusive breast milk is pure breast milk without additional food or additional fluids such as formula milk, water, etc. which is given to newborn babies before the baby is 6 months old. Woolwich massage has several benefits, including increasing prolactin and oxytocin reflexes (let down reflex), preventing blockages, increasing breast milk production and preventing inflammation or breast dams.. Objective: To determine out that giving Woolwich massage can lead to smooth breast milk production for postpartum mothers. Method: The method for writing this EBCR is by searching for evidence through data based Pubmed and Google Scholar with the publication year 2018-2023, 4 articles were obtained for critical review. Results: After evaluation before and after the Woolwich massage, the mother's breast milk production became smooth.. Conclusion: Giving Woolwich massage is effective in helping to facilitate breast milk production.

PENGARUH PEMBERIAN AROMATERAPI LAVENDER TERHADAP PENURUNAN NYERI PADA PERSALINAN KALA I

Lintang Novita — 2024-12-30

Background: Aromatherapy is a number of non-pharmacological techniques that can increase maternal comfort during labor and have an influence on effective coping with the labor experience. Purpose: to determine the effect of lavender aromatherapy on reducing pain in labor in the first stage. Methods: This study was conducted by searching the literature in several articles such as PubMed and Google Scholar according to predetermined criteria. Result: that in the first article there is an effect that occurs on the intensity of pain in labor in the first stage by providing lavender aromatherapy techniques, namely reduced pain between the latent phase and the early active phase is significantly lower in the group given aromatherapy. And in the second article there was also a decrease in labor pain during the active phase 1 after being given lavender aromatherapy with a pvalue = 0.000. Conclusion: there is an effect that occurs in reducing pain during the first stage of labor by providing lavender aromatherapy techniques.

EVIDENCE BASED CASE REPORT (EBCR) PENGARUH KOMPRES DINGIN TERHADAP PENURUNAN NYERI LUKA PERINEUM

Marha Putri Rachmatina — 2024-12-30

Perineal wounds are often causes pain and discomfort in patients, thereby reducing mobilization and inhibiting the wound healing process. Cold compresses have a local analgesic effect, reducing nerve sensitivity and lowering the pain threshold and reducing swelling. The aim of this research is to apply cold compresses as a method to reduce stitching wound pain during postpartum care. This research method involves searching for articles using e-data search engines based on Pubmed, Science Direct, and Google Scholar for 2013-2023 with the criteria of original articles with experimental methods. From the search results, 3 articles were obtained that met the criteria. Applying cold compresses during the postpartum period as a non-pharmacological intervention can be an alternative to reduce pain in perineal wounds.

The PENGARUH AKUPRESUR TITIK SP 6 TERHADAP NYERI PERSALINAN PADA KALA 1 FASE AKTIF

Nazma Nurmanisa — 2024-12-30

Childbirth is a physiological thing that everyone experiences. The 2017 Indonesian Demographic and Health Survey (SDKI) report shows that 17% of live births in the 5 years prior to the survey were from women 15-49 years old who were born via caesarean section. Fear is the main factor that causes pain in labor. Labor pain is one of the most intense types of pain that a woman may experience. Pain in women giving birth is normal because it is influenced by anatomical and physiological factors in the body. Controlling delivery pain is very important in order to progress the delivery process. One way is to use non-pharmacological techniques, namely the accupressure technique. The aim is to determine the effectiveness of applying acupressure to the SP 6 point to reduce pain in women giving birth. This research method uses article searches with the e-databased search engines Pubmed, and Google Scholar for 2013-2023 with the criteria of original articles, RCT, Systematic Review, and experimental methods. From the search results, 1 article was obtained that met the criteria. Applying pressure at the SP6 point can reduce the labor pain felt by the mother in labor, as well as reducing the duration of the active first stage of labor and the total length of labor.

PENGARUH SLOW STROKE BACK MASSAGE TERHADAP PENURUNAN KECEMASAN IBU NIFAS : EVIDENCE BASED CASE REPORT

Puspa Hapsari — 2024-12-30

Background: Labor pain is a physiological thing that happens to mothers during the labor process. During labor, the head in the pelvic cavity will shrink due to pressure on the dorsal nerve which causes pain to the mother during labor. Pain during childbirth is one of the factors that causes mothers to feel that giving birth is a painful. Differences in pain responses are also influenced by past experiences, anxiety and emotional tension. Through experience, a person develops various mechanisms to deal with pain. Emotional tension due to anxiety and fear of the birthing process can aggravate the perception of pain, and vice versa, pain induces fear which causes anxiety and can end in panic. Purpose : This Evidence Based Case Report is to prescribe differences in pain intensity during the first active phase of labor before and after application of counterpressure massage. Method : A search for articles regarding the effect of counterpressure massage was carried out on the Scholar e-database and the Garuda Portal. Result : The intensity of pain before being given Counterpressure massage obtained a value of 8 (very sever) while after being given Counterpressure massage obtained a value of 5 (moderate pain). Conclusion : counterpressure massage is effective in reducing labor pain.

EVIDENCE BASED CASE REPORT: PENGARUH AKUPRESUR TITIK LV4 DAN LI4 TERHADAP NYERI PERINEUM SETELAH PERSALINAN

Putri Dwi Salwa — 2024-12-30

Background: The application of acupressure can be used as a non=pharmacological technique for postpartum, acupressure can be applied to various point on the body. One of wivh is the points LV4 and LI4 points, these points are used to treat constipation, pain, swelling, and redness in genital area, as well as pain symptoms related to gynecological problems such as during chilbirth, and primary dysmenorrhea. The application of acupressure can be used as an analgesic technique to reduce pain due to perineal trauma (such as episiotomy and spontaneous rupture). Purpose: The effect of acupressure techniques at the LV4 and LI4 points on perineal suture pain after chilbirth. Method: This journal search method uses keywords, Boolean operators and E-data Based according to predetermined criteria. Result: There was an influence that occurred on the intensity of pain in perineal wounds by providing acupressure techniques two hours after delivery, namely reducing the pain of perineal suture wounds which began using the VAS instrument, namely 8 after 2 hours of intervention to 3. Conclusion: there is an effect on the intensity of pain in perineal wounds by providing acupressure techniques two hours after delivery, namely reducing the pain of perineal suture.

PENGARUH TEKNIK REBOZO TERHADAP PENURUNAN NYERI PERSALINAN (EVIDENCE BASED CASE REPORT)

Rinye Anggraeni Dewi — 2024-12-30

Background: Labor pain will affect discomfort physical and psychological conditions and labor process will interfere if not handled properly. In the labor when cervical dilation occurs, the greater the cervical dilation, the more frequent and painful the pain felt by the mother. Purpose: To reduce the use of drugs or pharmacological therapy, an intervention is carried out in the form of a non-pharmacological method using the rebozo technique. Method: This method uses the Evidence Based Case Report (EBCR). The article search was carried out using the PubMed, Google Scholar and Cochrane Library databases. The keywords used are appropriate and represent the population, rebozo technique, pain labor, pain relief and childbirth. The search for articles is limited and published in the last 10 years, articles with full text. 3 articles were obtained for use. Respondents will be measured for pain levels using the Numeric Rating Scale (NRS) and the Wong Baker Face Pain Rating Scale. Result: Before the intervention, the pain level was 6-7 with the severe pain category and after the intervention, the pain level was 4-5 with the moderate pain category. Conclusion: Rebozo technique intervention to reduce labor pain and delivery’s progress had a good effect.

EVIDENCE BASED CASE REPORT (EBCR) PENGARUH PENGGUNAAN BIRTH BALL DALAM MENGURANGI NYERI PERSALINAN

sarah nur — 2024-12-30

Background: In the labor process, the cervix opens and thins, and the head descends through the birth canal, uterine contractions occur with regular frequency and intensity that cause pain. Birth ball helps mothers in the first stage of labor, which aims to help the progress of labor that can be used in various positions, the use of birth ball in labor is useful in reducing pain and is a comfortable aid for laboring mothers.. Objective: Implementing the use of a birth ball can reduce the pain felt by mothers in the first stage of labor. Method: The method of writing this EBCR is by searching for evidence through data based on Pubmed and Google Scholar with publication years 2016-2023, 3 articles were obtained to be reviewed critically. Results: After an evaluation was carried out before and after the intervention using a birth ball, the mother in labor was able to reduce labor pain in the first stage. Conclusion: The use of a birth ball as a non-pharmacological intervention can be an alternative to reduce pain in mothers in the first stage of labor.

AKUPRESUR TITIK BL 32 TERHADAP NYERI PERSALINAN KALA I : EVIDANCE BASED CASE REPORT (EBCR)

Tri Riski Nur Khayuni — 2024-12-30

Background: Effective and painless labor pain management is an important strategy to encourage vaginal delivery and reduce the frequency of cesarean sections. One of the non-pharmacological methods that can be used to reduce labor pain is acupressure, with the BL32 point as one example. Objective: This Evidence Based Case Report (EBCR) aims to determine the effect of acupressure at the BL 32 point on first-stage labor pain. Method: This Evidence Based Case Report (EBCR) uses an evidence search method through PUBMED and Google Scholar based data which collects and analyzes previous research on the application of acupressure at the BL32 point during the first stage of labor with publication years 2014-2023. Results: There were 2 relevant articles according to the inclusion criteria. The author has applied acupressure at the BL32 point to labor pain in mowen giving birth in the first stage at the Garuda Health Center and it was found that the woman’s pain scale before the intervention was 8 while the pain scale after the intervention was 6. Literature studies, systematic reviews and meta-analyses also support that acupressure at the BL32 point can reduce the severity of labor pain in the first stage. Conclusion: The implementation of acupressure at the BL32 point has been proven to reduce pain during the first stage of labor.

THE EFFECT OF MORINGA OLEIFERA L EVIDENCE BASED CASE REPORT (EBCR): PENGARUH EKSTRAK DAUN KELOR (MORINGA OLEIFERA) TERHADAP PRODUKSI ASI DAN PENINGKATAN BERAT BADAN BAYI

Widi Putri — 2024-12-30

Background: Exclusive breastfeeding is giving only breast milk without giving other food and drinks to babies until they are 6 months old, except for medicine and vitamins. Failure to provide exclusive breastfeeding is influenced by various factors such as the assumption of low breast milk production, inadequate nutritional intake, low education, lack of knowledge, and lack of husband and family support. Purpose:To determine the effect of Moringa oleifera leaf extract on breast milk production in postpartum mothers and baby weight gain. Method: This Evidence Based Case Report (EBCR) was carried out by searching the literature in several articles such as Science Direct, PubMed, Garuda Portal, Google Scholar according to predetermined criteria. Results: An evaluation was carried out on the 3rd day of giving Moringa leaf extract capsules, there was an increase in breast milk volume of 38 ml and an increase in the baby's weight of 70 grams. Conclusion: There was an effect of giving Moringa oleifera leaf extract on breast milk production and the baby's weight gain.

PENGARUH KOMPRES ALOE VERA TERHADAP NYERI PEMBENGKAKAN PAYUDARA PADA IBU NIFAS: EVIDENCE BASED CASE REPORT

Zakiyyah Marsaa Khairani — 2024-12-30

Background: The postpartum is a period of adaptation where various problems arise, a problem that often arises during the postpartum period is breast engorgement. Breast engorgement occurs due to non-continuous breastfeeding so that remaining breast milk collects in the duct area. This occurs, among other things, because breast milk production increases, early breastfeeding is hampered, poor latching, less frequent expression of breast milk, and restrictions on breastfeeding time. According to the Indonesian Ministry of Health, breast engorgement often occurs in 10-20% of the population of postpartum mothers, which can make the situation worse if not treated properly. Objective: This evidence-based case report aims to determine the effect of aloe vera compresses on breast engorgement of postpartum mothers. Method: Evidence base case report (EBCR) through literature searches in several articles, namely Pubmed and Google Scholar. Results: The breasts were painful and swollen after being given treatment by applying aloe vera compresses for 20 minutes which was done 2-3 times a day for 7 days which reduced breast engorgement, seen from the decreasing Visual Analog Scale (VAS) assessment results. Conclusion: The application of non-pharmacological therapy, namely Aloe vera compress, contains anthraquinone containing aloin and emodin which can function as an analgesic so that it is effective in treating breast engorgement.

EFEKTIVITAS EFFLEURAGE HAND MASSAGE DENGAN AROMATERAPI LAVENDER UNTUK MENGURANGI KELELAHAN PADA IBU NIFAS

Zilca Nezima — 2024-12-30

Background: The postpartum period is a physiological thing experienced by every mother after giving birth and has an impact on physiological and psychological changes in postpartum mothers. One of the most common complaints experienced by postpartum mothers is fatigue. Symptoms of fatigue occur in around 64% of mothers in the postpartum period. It is reported that 75% of 10 postpartum mothers experience fatigue such as frequent yawning, stiff backs, unbalanced when standing and 25% say mothers have difficulty concentrating. Non-pharmacological therapy is needed to reduce fatigue in postpartum mothers, one of which is the effleurage hand massage technique with lavender aromatherapy. The combination of aromatherapy and massage in postpartum mothers can reduce the fatigue felt by the mother. Purpose: To determine the effectiveness of effleurage hand massage with lavender aromatherapy to reduce fatigue in postpartum mothers. Method: Case study on postpartum mothers using e-data based search engines Pubmed and Google Scholar with publication years 2017-2024. Result: After effleurage hand massage with lavender aromatherapy, the mother experienced mild fatigue. Conclusion: Effleurage hand massage with lavender aromatherapy is effective in reducing fatigue in postpartum mothers.

EVIDENCE BASED CASE REPORT (EBCR) : PENGARUH COUNTER PRESSURE MASSAGE PADA PENURUNAN NYERI PERSALINAN FASE AKTIF

Nadya Puspa Maharani — 2024-12-30

Anemia in Post Partum

Rahmi Afria — 2024-12-30

Anemia is a complication that is postpartum mothers. The main cause is bleeding during the birth process. In 2023, 92 (8.47%) of the 1085 mothers who gave birth spontaneously will handle post-partum hemorrhage at the Sekarwangi Hospital. The aim of this midwifery care is to apply Post Partum Midwifery Care to Mrs. W aged 30 years P3A1 with moderate anemia at Sekarwangi Hospital.The method in preparing this assignment report is a case study. The form of documentation is SOAP. Data collection techniques obtained through interviews, physical and laboratory examination observations, the mother gave birth to her fourth child 8 hours ago, the mother had a miscarriage once, the mother complained of feeling weak, dizzy, her eyes were dizzy. Examination of vital signs showed blood pressure 90/60 mmHg, pulse 92x/m, respiration 21x/m, temperature 36.5 C, face, conjunctiva and gums pale, genital examination found blood output 50cc, and supporting examination with hemoglobin examination with results of 7.7 gr/dl. The analysis that can be confirmed is "Mrs. W, 30 years old P3A1 post partum 8 hours with moderate anemia." The management carried oral therapy, amoxicillin and Fe tablets, blood transfusions, observing the general condition, bleeding, uterine contractions, nutritional counseling. Conclusion from midwifery care for moderate anemia is that it can be carried out well with cooperation between the client and the author. be able to carry out the recommendations suggested by the midwife, from that the midwife is expected treat anemia in appropriate service standards and midwife authority.

Reproductive Health Care in Adolescents with Vaginal Discharge: A Case Report"

siti Asiva — 2024-12-31

Vaginal discharge is a fluid released from the genital area, excluding blood, and often occurs due to poor hygiene in the genital area. Pathological vaginal discharge is characterized by milky white, thick fluid accompanied by itching and is commonly caused by bacterial, viral, fungal, or parasitic infections. Over the past three months, 28 cases of pathological vaginal discharge were reported out of 53 reproductive health complaints at Parung Community Health Center. This report aims to apply midwifery care for Miss R, an 18-year-old patient, with pathological vaginal discharge at Parung Community Health Center.The method used a case report with a SOAP approach (Subjective, Objective, Analysis, Management). Data were collected through interviews, observations, physical examinations, documentation studies, and literature reviews. The analysis showed that Miss R experienced pathological vaginal discharge caused by fungal infection (Candida albicans), triggered by unhealthy habits such as wearing non-absorbent underwear and inadequate genital hygiene.Management included pharmacological therapy with a single dose of oral oral metronidazole 500mg and antifungal cream to relieve itching, as well as non-pharmacological education on genital hygiene, using cotton underwear, and avoiding scented hygiene products. Care was provided over 10 days, resulting in significant improvement in symptoms.In conclusion, the management of pathological vaginal discharge tailored to the patient’s needs produced optimal results. Education for the patient and her family is essential to detect signs of pathological vaginal discharge and prevent recurrence in the future.

MIDWIFERY CARE FOR PREGNANT WOMEN WITH CONDYLOMA ACUMINATA: A CASE REPORT

Dwi Nur Anggraini — 2024-12-31

Teenage pregnancy can cause several risks to both the mother and the fetus, for the fetus there can be complications of premature birth and LBW. In the mother there can be bleeding, perineal tears and prolonged labor. In 2022, as many as 12.8 million mothers who gave birth were aged 15-19 years. In this case, the mother with a young pregnancy aged 18 years accompanied by condyloma acuminata disease has the potential for respiratory problems, vocal cord damage, and blindness in the eyes of babies infected with HPV. The methods used in compiling the Report are case reports, midwifery management approaches and SOAP documentation (Subjective, Objective, Analysis, Management). Data collection techniques use interview techniques, physical examinations, observations, documentation studies, and literature studies. Analysis in this case Mrs. D,18 years old pregnant 31 weeks with condyloma acuminata, care provided in collaboration with a SPog doctor by administering 80-90% trichloroacetic acid ointment and 0.1% gentamicin sulfate ointment therapy, education on STDs and sexuality, after two months of care condyloma acuminata resolved. The conclusion of the results of care is in accordance with the standards of pregnancy care, but there are obstacles, namely the husband who is not cooperative. The suggestion in this case is to invite the husband to undergo treatment so that the sexually transmitted disease in this couple is resolved.

Midwifery Care for Nn. T With Primary Dysmenorrhea: Case Report

Theyhana Qory Tila Thabrani — 2024-12-31

The menstrual disorder that teenagers often experienced was dysmenorrhoea, so it must receive appropriate treatment because it is related to one of the causes of teenage girls' absence from school. The prevalence of primary type dysmenorrhea in Indonesia is 6 times higher than secondary type dysmenorrhea. In reproductive health care, midwives detect signs and symptoms and provide education to clients. The method used in writing this report is a case report. Data collection techniques from interviews, physical examination, observation, documentation studies, and literature studies. The report documentation use SOAP. The results of the research obtained subjective data from Mrs. T came on the second day of menstruation with complaints of pain in the lower abdomen, pain in the waist and hips every time during menstruate on days 1-3. Objective data showed a tenderness in the lower abdomen. The analysis was Mrs. T 14 years old with primary dysmenorrhoea. The intervention provided include measuring the pain scale using the NRS instrument with NRS score 3, education, dysmenorrhoea exercises, giving Fe, as well as management principle of pain reduction. The conclusion from the intervention given was that there was a decrease in the scale of pain during menstruation. However, there is a gap because there are no fixed procedures for treating dysmenorrhoea. The advice given to Mrs. T was to carry out the services provided to reduce pain during menstruation, suggestions for health services and midwives to improve the quality of providing education regarding reproductive health in accordance with established procedures.

THE EFFECT OF EDUCATION ON PUBLIC KNOWLEDGE ABOUT ANTI-TUBERCULOSIS DRUGS AT THE GEGER HEALTH CENTER

Riyadatus Solihah — 2024-12-31

TB is a very contagious disease caused by Mycobacterium . Mycobacterium tuberculosis is the most common infection which mostly attacks the lungs. Anti-Tuberculosis Drugs (ATD) are the most important component in the treatment of tuberculosis. Tuberculosis treatment is one of the most efficient to prevent the further spread of tuberculosis and include antibiotic drugs which function in various ways to kill tuberculosis bacteria. The purpose of study is to determine the effect of education about Anti-Tuberculosis Drugs (ATD) on public knowledge at the Geger Health Center.

This research used observational analytics using a cross sectional approach, the population was 78 respondents with a sample of 65 respondents. The sampling technique was non-probability sampling with the purposive sampling method. The research was conducted at the Geger health center in April 2024. The data collection used questionnaires. Data analysis was univariate and bivariate using the paired t-test. The results of the research before being given education (pretest) 30 (46.2%) respondents were in the poor category, while after given education (posttest) 53 (81.5%) were in the good category. Based on the results of statistical using t-test, the p-value (0.000) < α (0.05) was obtained, which means there was an effect of education on public knowledge about anti-tuberculosis drugs at Geger health centers. It was concluded that there was an increase in public knowledge about anti-tuberculosis drugs at Geger health centers after being given education. The suggestion for future researchers is to add variables that can influence the attitude of Tuberculosis patients towards treatment.