Development of an iron diagnostic method using Malaka fruit based on digital imaging
DOI:
https://doi.org/10.34011/jmp2k.v36i1.4045Keywords:
digital imaging, gallic acid, iron, malacca fruit, smartphoneAbstract
Background: Iron (Fe) is a geochemically active element that needs to be monitored because excessive levels in water can reduce water quality and pose health risks. WHO sets a safe limit of Fe in drinking water at 0.3 mg/L. This condition demands an accurate, simple, and applicable Fe detection method without complex instruments.
Objective: This study developed a digital imaging-based Fe(III) detection method using a natural reagent, malacca fruit extract, which is rich in gallic acid and forms a blue Fe-gallic acid complex.
Methods: The study design included laboratory experiments involving gallic acid extraction, optimization of the Fe(III): extract ratio, optimization of measurement time, and evaluation of linearity, precision, accuracy, LoD, LoQ, and t-test against the UV-Vis spectrophotometry method.
Results: The results showed an optimum Fe(III): extract ratio of 1:2, with reaction stability up to the 5th minute. A linear relationship was obtained in the range of 0.1–2 ppm (R² = 0.9969), with an LoD of 0.1377 ppm and a LoQ of 0.4589 ppm. The %RSD values of 2.34–3.82% indicate good precision, while the accuracy ranges from 92.76–111.2%. A t-test confirmed that the digital imaging results were not significantly different from those of UV-Vis spectrophotometry.
Conclusion: Overall, this method offers a portable, economical, and environmentally friendly analytical approach for Fe(III) detection and provides a basis for the development of digital application-based diagnostic systems using natural reagents.
References
[1] X. Xia, Y. Teng, and Y. Zhai, “Biogeochemistry of Iron Enrichment in Groundwater: An Indicator of Environmental Pollution and Its Management,” Sustainability, vol. 14, no. 12, p. 7059, 2022.
[2] E. A. M. Abed, K. A. N. Alaboudi, M. H. H. Abbas, T. M. S. Attia, and A. A. Abdelhafez, “Iron Contamination in Groundwater: Risk Assessment and Remediation Techniques in Egypt’s New Valley,” Water, vol. 16, no. 13, p. 1834, 2024.
[3] T. Harmawan, “Analisa Besi pada Serum Penderita Diabetes Mellitus yang Berobat di Rumah Sakit (RS) Balimbingan PTPN IV Pematang Siantar,” Quim. J. Kim. Sains dan Terap., vol. 1, no. 2, pp. 24–7, 2019.
[4] N. Jaikrajang, S. Kruanetr, D. Harding, and P. Rattanakit, “A simple flow injection spectrophotometric procedure for iron (III) determination using Phyllanthus emblica Linn. as a natural reagent,” Spectrochim. Acta Part A Mol. Biomol. Spectrosc., vol. 204, pp. 726–34, 2018.
[5] P. Rattanakit and R. Maungchang, “Determining iron (III) concentration in a green chemistry experiment using Phyllanthus emblica (Indian Gooseberry) extract and spectrophotometry,” J. Chem. Educ., vol. 96, no. 4, pp. 756–60, 2019.
[6] F. T. Ferreira, K. A. Catalão, R. B. Mesquita, and A. O. Rangel, “New Microfluidic Paper-Based Analytical Device For Iron Determination In Urine Samples,” Anal. Bioanal. Chem., vol. 413, pp. 7463–7472, 2021.
[7] T. Ozer, C. McMahon, and C. S. Henry, “Advances in paper-based analytical devices,” Annu. Rev. Anal. Chem., vol. 13, no. 1, pp. 85–109, 2020.
[8] S. Dortez, A. G. Crevillen, and A. Escarpa, “Integrated Calibration And Serum Iron In Situ Analysis Into An Array Microfluidic Paper-Based Analytical Device With Smartphone Readout,” Talanta, vol. 253, p. 123914, 2023.
[9] R. K. Bhattarai, S. Pudasaini, M. Sah, B. B. Neupane, and B. Giri, “Handmade Paper as a Paper Analytical Device for Determining the Quality of an Antidiabetic Drug,” ACS Omega, vol. 7, no. 16, p. 14074−14081, 2022, doi: 10.1021/acsomega.2c00633.
[10] G. Rateni, P. Dario, and F. Cavallo, “Smartphone-Based Food Diagnostic Technologies : A Review,” Sensors, vol. 17, no. 14, p. 1453, 2017, doi: 10.3390/s17061453.
[11] A. Wardani, G, L. Abiya, S, and F. Setiawan, “Analysis of the Lead on Lip Tint Cosmetics on the Market Using UV-Vis Spectrophotometry Method,” Edu Chem. (Jurnal Kim. Dan Pendidikan), vol. 5, no. 1, p. 87, 2020.
[12] K. et al Shrivas, “Smartphone coupled with paper-based chemical sensor for on-site determination of iron(III) in environmental and biological samples,” Sensors Actuators B Chem., vol. 304, p. 127404, 2020.
[13] O. T. Mahardani and L. Yuanita, “Efek Metode Pengolahan Dan Penyimpanan Terhadap Kadar Senyawa Fenolik Dan Aktivitas Antioksidan,” Unesa J. Chem., vol. 10, no. 1, pp. 64–78, 2021, doi: 10.26740/ujc.v10n1.p64-78.
[14] Rahmawati and Istiqomah. et al, “Gallic acid: A promising bioactive agent for food preservation and sustainable packaging development,” Case Stud. Chem. Environ. Eng., vol. 10, p. 100776, 2024.
[15] Badan Standardisasi Nasional, “SNI 6989.4: 2009 Air dan Limbah- Bagian 4: Cara Uji Besi (Fe) secara Spektrofotometri Serapan Atom (SSA)-nyala,” Jakarta, 2009.
[16] Badan Standardisasi Nasional, “SNI ISO/IEC 17025:2017 Kompetensi Laboratorium,” Jakarta, 2017.
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