A COMBINATION OF APPLE AND CINNAMON EXTRACTS REDUCES BLOOD GLUCOSE AND INCREASES INSULIN LEVELS OF T2DM MODEL RATS
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Abstract
Prevalensi penyandang penyakit diabetes melitus (DMT2) terus meningkatan akibat kenaikan kadar glukosa darah dan resisten insulin oleh sel β pancreas. Hiperglikemia pada DMT2 dapat menyebabkan stress oksidatif. Pemberian kombinasi ekstrak apel dan kayu manis memiliki potensi untuk menurunkan kadar glukosa darah karena mengandung senyawa flavonoid dan antioksidan yang mencegah stress oksidatif. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian Kombinasi Ekstrak Apel dan Kayu manis (KEAK) terhadap kadar GDP dan insulin pada tikus DMT2. Jenis penelitian ini adalah eksperimental laboratorik dengan rancangan randomized pre-post test control group design. Penelitian ini menggunakan 36 tikus Rattus norvegicus Jantan, dibagi menjadi 6 kelompok: KN tikus dikondisikan normal, K- tikus DMT2 tanpa perlakuan, K+ tikus DMT2 yang diberikan obat metformin, kelompok KP1 (9,14+45 mg/200 gramBB), KP2 (18,36+30 mg/200 gramBB) dan KP3 (27,42+15mg/200gramBB) yaitu tikus DMT2 yang di berikan KEAK selama 21 hari. Analisis data menggunakan uji One-way Anova. Setelah 21 hari intervensi KP1 mengalami penurunan kadar GDP (Δ -177,88 ± 4,61) dan kenaikan kadar insulin tertinggi (Δ 81,83 ± 9,37) dibandingkan dengan perlakuan yang lain. KP2 mengalami penurunan kadar GDP (Δ -167,41 ± 4,13) dan kenaikan kadar insulin (Δ 62,82 ± 9,37) yang hampir sama dengan kelompok perlakuan yang diberikan obat metformin (K+) dengan penurunan kadar GDP (Δ -167,80 ± 4,96) dan kadar insulin (Δ 56,23 ± 8,54). Pemberian kombinasi ekstrak apel dan kayu manis berpengaruh dalam menurunkan kadar GDP dan meningkatkan kadar insulin. Kombinasi ekstrak apel dan kayu manis dapat dipertimbangkan untuk menurunkan GDP dan meningkatkan kadar insulin.
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References
WHO, “Diabetes,” 2023. https://www.who.int/news-room/fact-sheets/detail/diabetes
IDF, “International Diabetes Federation Atlas 10th edition,” Diabetes Res. Clin. Pract., vol. 102, no. 2, pp. 147–148, Nov. 2021, doi: 10.1016/j.diabres.2013.10.013.
PERKENI, Pedoman pengolahan dan pencegahan diabetes melitus tipe 2 dewasa di Indonesia. PB PERKENI, 2019. doi: 10.7748/NM.2020.E1928.
IDF, 9th IDF Diabetes Atlas. 2019.
K. R. W. I. M. K. W. P. A. Suputra, “Diabetes Melitus Tipe 2 : Faktor Risiko , Diagnosis , Dan Tatalaksana,” vol. 1, no. 2, pp. 114–120, 2021.
M. P. B. Gumantara and R. Z. Oktarlina, “Perbandingan Monoterapi dan Kombinasi Terapi Sulfonilurea-Metformin terhadap Pasien Diabetes Melitus Tipe 2,” Majority, vol. 6, no. 1, pp. 55–59, 2017.
PERKENI, “Pedoman Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 Dewasa di Indonesia 2021,” Glob. Initiat. Asthma, p. 46, 2021, [Online]. Available: www.ginasthma.org.
N. Namazi, S. Esmaeili, S. Ahmadikhatir, F. Razi, E. Nasli-Esfahani, and B. Larijani, “Nutrition and Diet Therapy in Diabetes Mellitus: A Roadmap based on available evidence.,” J. Diabetes Metab. Disord., vol. 20, no. 2, pp. 1913–1918, Dec. 2021, doi: 10.1007/s40200-021-00876-2.
Fitriani, Akhmad, and Lestariana, “Efek kuersetin terhadap kadar glukosa darah puasa pada tikus diabetes melitus tipe 2 yang diinduksi dengan streptozotocin-nicotinamide,” vol. 6, no.2, pp. 104-111, Mei-Agustus 2014.
Munadia and V. Aulianshah, “Perbandingan Aktivitas Antioksidan Jus dan Infused Water Apel Hijau (Malus sylvestris Mill.),” J. Ilm. Farm. Simplisia, vol. 1, no. 1, pp. 8–11, 2021.
M. L. T. da Silva, M. A. S. Bernardo, J. Singh, and M. F. de Mesquita, Beneficial uses of cinnamon in health and diseases: An interdisciplinary approach. Elsevier Inc., 2019. doi: 10.1016/B978-0-12-813148-0.00033-5.
L. Mamluk et al., “Fruit and vegetable intake and risk of incident of type 2 diabetes: results from the consortium on health and ageing network of cohorts in Europe and the United States (CHANCES),” Physiol. Behav., vol. 176, no. 1, pp. 139–148, 2017, doi: 10.1038/ejcn.2016.143.Fruit.
N. Yuliwati and R. F. Nugroho, “The Potential of Strawberry , Rome Beauty Apple , and New Combination on Fasting Blood as Supporting Diet Therapy in Patients with Type II Diabetes Mellitus,” Glob. Med. Heal. Commun. Online, vol. 9, no. April, pp. 69–75, 2021.
P. Ansari et al., “Therapeutic Potential of Quercetin in the Management of Type-2 Diabetes Mellitus,” MDPI, vol. 12, no. 8, pp. 1–18, 2022.
R. Dhanya, “Quercetin for managing type 2 diabetes and its complications, an insight into multitarget therapy,” Biomed. Pharmacother., vol. 146, pp. 112560, 2022, doi: https://doi.org/10.1016/j.biopha.2021.112560.
P. Ansari et al., “Therapeutic Potential of Quercetin in the Management of Type-2 Diabetes Mellitus.,” Life (Basel, Switzerland), vol. 12, no. 8, Jul. 2022, doi: 10.3390/life12081146.
L. K. Khotimah, D. Iktianti, and J. Susilo, “Perbandingan Efek Pemberian Jus Buah Apel Hijau (Mallus sylvestris Mill.) dan Jus Buah Apel Merah (Mallus domestica Borkh.) Terhadap Gangguan Toleransi Glukosa Darah Pada Tikus Putih Jantan Akibat Efek Samping Deksametason,” vol. 54, p. 2016, 2016.
M. Luthfi, D. Norviatin, and A. Sutisna, “Perbandingan Efektivitas Ekstrak Kayu Manis ( Cinnamomum cassia ) Dengan Metformin Terhadap Glukosa Darah Tikus Galur Wistar,” … J. Kedokt. Kesehat., vol. 5, no. 2, 2019.
Syafriani and B. Verawati, “Pengaruh Ekstrak Kayu Manis Terhadap Penurunan Kadar Gula Darah Pada Penderita Dm Tipe Ii Di Desa Kumantan Wilayah Kerja Puskesmas Bangkinang Kota,” J. Ners, vol. 1, no. 2, pp. 90–95, 2017, doi: 10.31004/jn.v1i2.120.
A. Landani and E. Kurniawaty, “Pengaruh Pemberian Kayu Manis ( Cinnamomum cassia ) Terhadap Penurunan Gula Darah Pada Penderita Diabetes Melitus The Effect of Giving a Cinnamon ( Cinnamomum cassia ) to The Blood Sugar Decrease in People with Diabetes Melitus Type 2,” J urnal Agromedicine Unila, vol. 5, no. 1, pp. 1–5, 2018.
E. M. Ayu Prahartini Nur Sahid, “Pengaruh Bubuk Daun Kenikir (Cosmos Caudatus) Terhadap Kadar Glukosa Darah Tikus Diabetes Diinduksi Streptozotocin,” J. Nutr. Coll., vol. 5, no. 2, pp. 51–57, 2016.
D. A. Ghassana, A. P. W. Marhendra, and A. E. P. Haskito, “The preventive effect of Rome beauty (Malus sylvestris Mill) apple peel’s extract towards the malondialdehyde (MDA) levels of rat (Rattus norvegicus) stomach exposed to plumbum acetate,” J. Phys. Conf. Ser., vol. 1430, no. 1, pp. 12016, 2020, doi: 10.1088/1742-6596/1430/1/012016.
M. Luthfi, D. Norviatin, and A. Sutisna, “Perbandingan Efektivitas Ekstrak Kayu Manis (Cinnamomum cassia) Dengan Metformin Terhadap Glukosa Darah Tikus Galur Wistar,” … J. Kedokt. Kesehat., vol. 5, no. 2, 2019, [Online]. Available: http://jurnal.ugj.ac.id/index.php/tumed/article/view/4201
B. L. Furman, “Streptozotocin-Induced Diabetic Models in Mice and Rats,” Curr. Protoc., vol. 1, no. 4, p. e78, 2021, doi: https://doi.org/10.1002/cpz1.78.
I. Setyawati, “Ekstrak Etanol Biji Kluwih (Artocarpus camansi) sebagai Agen Antihiperglikemia pada Tikus Model Diabetes,” J. Multidisiplin Indones., vol. 2, no. DM, pp. 886–896, 2023, [Online]. Available: file:///C:/Users/HP/Downloads/289-Article Text-2501-1-10-20230628.pdf
I. Krissanti, R. Hanifa, and R. G. Dwiwina, “Efektivitas dan Pengaruh Kombinasi Anestesi Ketamine-Xylazine pada Tikus (Rattus norvegicus),” Gunung Djati Conf. Ser., vol. 18, pp. 245–252, 2023, [Online]. Available: https://conference.uinsgd.ac.id/index.php/Telp/Fax:
J. Zhu, H. Chen, Z. Song, X. Wang, and Z. Sun, “Effects of Ginger (Zingiber officinale Roscoe) on Type 2 Diabetes Mellitus and Components of the Metabolic Syndrome: A Systematic Review and Meta-Analysis of Randomized Controlled Trials,” Evidence-based Complement. Altern. Med., vol. 2018, pp. 5692962, 2018, doi: 10.1155/2018/5692962.
A. N. Wijayanti, “Efektivitas Kapsul Ekstrak Buah Mengkudu ( Morinda citrifolia L.) Terhadap Penurunan Kadar Glukosa Darah Mencit ( Mus musculus L.),” J. Kesehat. Pharmasi, vol. IV, no. 1, pp. 68–73, 2022.
N. Z. Ramadhani, Y. Tursinawati, and D. Mustika, “Pengaruh Pemberian Beras Ketan Hitam (Oryza sativa L . Var glutinosa) terhadap Perubahan Kadar Gula Darah Tikus Wistar yang Diinduksi Streptozotocin_NA,” vol. 4, no. 1, 2022.
I. Saadane, T. Ashraf, T. Ali, and N. Lessan, “Diabetes and Ramadan: Utility of flash-glucose monitoring derived markers of glycaemic control and comparison with glycosylated haemoglobin.,” Diabetes Res. Clin. Pract., vol. 153, pp. 150–156, Jul. 2019, doi: 10.1016/j.diabres.2019.05.020.
S. Fadlilah, A. Sucipto, F. Khasanah, D. Setiawan, and N. Hamdani Rahil, “Dragon Fruit (Hylocereuspolyrhizus) Effectively Reduces Fasting Blood Sugar Levels and Blood Pressure on Excessive Nutritional Status,” Pakistan J. Med. Heal. Sci., vol. 14, no. 2, pp. 1405–1412, 2020.
U. Asmat, K. Abad, and K. Ismail, “Diabetes mellitus and oxidative stress-A concise review.,” Saudi Pharm. J. SPJ Off. Publ. Saudi Pharm. Soc., vol. 24, no. 5, pp. 547–553, Sep. 2016, doi: 10.1016/j.jsps.2015.03.013.
E. Mann and M. D. Bellin, “Secretion of Insulin in Response to Diet and Hormones,” Pancreapedia, vol. 1, no. 1, pp. 1–16, 2016, doi: 10.3998/panc.2016.3.
H. Alkhalidy, Y. Wang, and D. Liu, “Dietary Flavonoids in the Prevention of T2D: An Overview.,” Nutrients, vol. 10, no. 4, Mar. 2018, doi: 10.3390/nu10040438.
K. Farrokhfall, A. Khoshbaten, S. Zahediasl, H. Mehrani, and N. Karbalaei, “Improved islet function is associated with anti-inflammatory, antioxidant and hypoglycemic potential of cinnamaldehyde on metabolic syndrome induced by high tail fat in rats,” J. Funct. Foods, vol. 10, pp. 397–406, 2014, doi: https://doi.org/10.1016/j.jff.2014.07.014.
G. Wilcox, “Insulin and insulin resistance.,” Clin. Biochem. Rev., vol. 26, no. 2, pp. 19–39, May 2015.
A. B. Medagama, “The glycaemic outcomes of Cinnamon, a review of the experimental evidence and clinical trials.,” Nutr. J., vol. 14, p. 108, Oct. 2015, doi: 10.1186/s12937-015-0098-9.