PENGARUH EKSTRAK ETANOL DAUN RAMANIA TERHADAP INSULIN DAN GDP PADA TIKUS OBESITAS DM TIPE 2
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Abstrak
Obesity is a major nutritional issue that can trigger insulin resistance, hyperlipidemia, and contribute to Type 2 Diabetes Mellitus (T2DM). Ramania leaves, rich in antioxidants such as flavonoids, have potential as antidiabetic and anti-obesity agents. This study aimed to evaluate the effects of ethanol extract of Ramania leaves on insulin levels and fasting blood glucose (FBG) in obese rats with T2DM. The research employed a true experimental laboratory method with a Randomized Pre-Posttest Control Group design to examine the effects of Ramania leaf ethanol extract on insulin levels and FBG in male Wistar rats (Rattus norvegicus) with obesity-induced T2DM. The study was conducted over six weeks at the PSPG Laboratory of Gadjah Mada University. Subjects were divided into six groups: KNo (normal control, non-obese model group), KN (negative control, obese T2DM rats), KP (positive control, obese T2DM rats treated with metformin), and P1, P2, and P3 (obese T2DM rats treated with Ramania leaf extract at doses of 150,300, and 600 mg/kg BW for 14 days). Obesity and T2DM induction were conducted using a high-fat diet for 14 days, followed by streptozotocin administration at 45 mg/kg BW/day for three days. ANOVA testing revealed significant differences between groups (P<0.000), demonstrating the effectiveness of Ramania leaf ethanol extract (EEDR). Treatment with EEDR significantly increased insulin levels across all treatment groups (KP,P1,P2,P3) compared to the negative control (KN), highlighting the potential of EEDR in insulin regulation for obese T2DM rats. Ramania leaf extract showed significant potential in enhancing insulin levels and reducing FBG levels.
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Referensi
E. Kumalasari, S. Yugo, Y. R. Maulida, and D. R. Febrianty, “Pengaruh Pemberian Ekstrak Etanol Daun Ramania (Bouea macrophylla Griffith) Terhadap Penurunan Kadar Gula Darah Mencit Putih (Mus muscullus) Yang Diinduksi Aloksan,” Journal Current Pharmaceutical Sciences, vol. 2, no. 2, pp. 2598–2095, 2019.
S. Aryzki, S. Budi, and Y. Yanti, “Uji Aktivitas Antioksidan Ekstrak Etanol 96% Daun RAMANIA (Bouea macrophylla Griffith.) dari Daerah Kalimantan Selatan dengan Metode DPPH,” Jurnal Insan Farmasi Indonesia, vol. 6, no. 3, pp. 194–201, Dec. 2023, doi: 10.36387/jifi.v6i3.1692.
S. Aryzki, S. Budi, and Y. Yanti, “Uji Aktivitas Antioksidan Ekstrak Etanol 96% Daun RAMANIA (Bouea macrophylla Griffith.) dari Daerah Kalimantan Selatan dengan Metode DPPH,” Jurnal Insan Farmasi Indonesia, vol. 6, no. 3, pp. 194–201, Dec. 2023, doi: 10.36387/jifi.v6i3.1692.
M. M. Habeebulla, “In vitro Antioxidant and Antidiabetic Activities of the Bark Extracts of Kandelia candel (L.) Druce,” Asian Journal of Chemistry, vol. 30, no. 8, pp. 1717–1722, 2022.
F. Milita, S. Handayani, and B. Setiaji, “Kejadian Diabetes Mellitus Tipe II pada Lanjut Usia di Indonesia (Analisis Riskesdas 2018),” Jurnal Kedokteran dan Kesehatan, vol. 17, no. 1, p. 9, 2021, doi: 10.24853/jkk.17.1.9-20.
H. D. McIntyre, P. Catalano, C. Zhang, G. Desoye, E. R. Mathiesen, and P. Damm, “Gestational diabetes mellitus,” Nature Reviews Disease Primers, vol. 5, no. 1, p. 47, 2019, doi: 10.1038/s41572-019-0098-8.
M. Safaei, E. A. Sundararajan, M. Driss, W. Boulila, and A. Shapi’i, “A systematic literature review on obesity: Understanding the causes & consequences of obesity and reviewing various machine learning approaches used to predict obesity,” Computers in Biology and Medicine, vol. 136, no. August, p. 104754, 2021, doi: 10.1016/j.compbiomed.2021.104754.
O. A. Ojo et al., “Antidiabetic activity of avocado seeds (Persea americana Mill.) in diabetic rats via activation of PI3K/AKT signaling pathway,” Scientific Reports, vol. 12, no. 1, pp. 1–17, 2022, doi: 10.1038/s41598-022-07015-8.
S. D. Suryanti, A. T. Raras, C. Y. Dini, and A. H. Ciptaningsih, “Hubungan Indeks Masa Tubuh Dengan Kadar Gula Darah Puasa Pada Pasien Diabetes Melitus Tipe 2,” Poltekita: Jurnal Ilmu Kesehatan, vol. 13, no. 2, pp. 86–90, 2019.
M. Safaei, E. A. Sundararajan, M. Driss, W. Boulila, and A. Shapi’i, “A systematic literature review on obesity: Understanding the causes & consequences of obesity and reviewing various machine learning approaches used to predict obesity,” Computers in Biology and Medicine, vol. 136, no. August, p. 104754, 2021, doi: 10.1016/j.compbiomed.2021.104754.
Hariani, Abd. Hady, Nuraeni Jalil, and Surya Arya Putra, “Hubungan Lama Menderita Dan Komplikasi Dm Terhadap Kualitas Hidup Pasien Dm Tipe 2 Di Wilayah Puskesmas Batua Kota Makassar,” Jurnal Ilmiah Kesehatan Diagnosis, vol. 15, no. 1, pp. 56–63, 2020, doi: 10.35892/jikd.v15i1.330.
T. N. V. Nguyen Huyen Thi Thanh; Khuong, Long Quynh; van der Ploeg, Ingeborg; Sundberg, Carl Johan, “Positive Effects of Physical Activity on Prescription on Glycemic Control, Fitness, and Quality of Life in Newly Diagnosed Type 2 Diabetic Patients,” Horm Metab Res, vol. 55, no. 09, pp. 617–624, 2023, doi: 10.1055/a-2144-7236.
O. T. Lahamado, S. M. Sabang, and K. Mustapa, “Tamarind ( Tamarindus indica L . ) Leaves Extracts as Antidiabetic,” Jurnal Akademika Kimia, vol. 6, no. 1, pp. 1–6, 2017.
S. J. Maleki, J. F. Crespo, and B. Cabanillas, “Anti-inflammatory effects of flavonoids,” Food Chemistry, vol. 299, no. March, 2019, doi: 10.1016/j.foodchem.2019.125124.
J. C. J. M. D. S. Menezes and M. F. Diederich, “Bioactivity of natural biflavonoids in metabolism-related disease and cancer therapies,” Pharmacological Research, vol. 167, no. December 2020, 2021, doi: 10.1016/j.phrs.2021.105525.
F. Milita, S. Handayani, and B. Setiaji, “Kejadian Diabetes Mellitus Tipe II pada Lanjut Usia di Indonesia (Analisis Riskesdas 2018),” Jurnal Kedokteran dan Kesehatan, vol. 17, no. 1, p. 9, 2021, doi: 10.24853/jkk.17.1.9-20.
L. Triana and M. Salim, “Perbedaan Kadar Glukosa Darah 2 Jam Post Prandial,” Jurnal Laboratorium Khatulistiwa, vol. 1, no. 1, p. 51, 2017, doi: 10.30602/jlk.v1i1.97.
Q. P. Irawan, K. D. Utami, S. Reski, and Saraheni, “Hubungan Indeks Massa Tubuh (IMT) dengan Kadar HbA1c pada Penderita Diabetes Mellitus Tipe II di Rumah Sakit Abdoel Wahab Sjahranie,” Formosa Journal of Science and Technology, vol. 1, no. 5, pp. 459–468, 2022, doi: 10.55927/fjst.v1i5.1220.
R. Salto, D. Gir, C. Ortiz-moral, M. Manzano, and J. M. Lopez-pedrosa, “Dietary Complex and Slow Digestive Carbohydrates,” 2020.
I. Saputra, F. Esfandiari, E. Marhayuni, and M. Nur, “Indeks Massa Tubuh dengan Kadar Hb-A1c pada Pasien Diabetes Melitus Tipe II,” Jurnal Ilmiah Kesehatan Sandi Husada, vol. 12, no. 2, pp. 597–603, 2020, doi: 10.35816/jiskh.v12i2.360.
T. N. V. Nguyen Huyen Thi Thanh; Khuong, Long Quynh; van der Ploeg, Ingeborg; Sundberg, Carl Johan, “Positive Effects of Physical Activity on Prescription on Glycemic Control, Fitness, and Quality of Life in Newly Diagnosed Type 2 Diabetic Patients,” Horm Metab Res, vol. 55, no. 09, pp. 617–624, 2023, doi: 10.1055/a-2144-7236.
B. Mulyati and R. S. Panjaitan, “Studi Penambatan Molekul Flavonoid Pada Reseptor α-Glukosidase menggunakan PLANTS,” Jurnal Kimia Mulawarman, vol. 18, no. 2, p. 68, 2021, doi: 10.30872/jkm.v18i2.1004.
M. M. Habeebulla, “In vitro Antioxidant and Antidiabetic Activities of the Bark Extracts of Kandelia candel (L.) Druce,” Asian Journal of Chemistry, vol. 30, no. 8, pp. 1717–1722, 2022.
F. Nasution, A. Andilala, and A. A. Siregar, “Faktor Risiko Kejadian Diabetes Mellitus,” Jurnal Ilmu Kesehatan, vol. 9, no. 2, p. 94, May 2021, doi: 10.32831/jik.v9i2.304.
R. Alfiqi, S. S. Rahardjo, and L. Wijayanti, “The Effect of Ethanol Ants Nest Extract on Profil Lipid Mice Model Obesity with Type 2 Diabetes Program of Nutrition Science , Sebelas Maret University , Surakarta , Central Java , Indonesia 2 Department of Pharmacology , Faculty of Medicine , Sebelas Mar,” Jurnal Ilmu Kesehatan, vol. 17, no. 3, pp. 548–554, 2023.
R. Alfiqi, S. S. Rahardjo, and L. Wijayanti, “The Effect of Ethanol Ants Nest Extract on Profil Lipid Mice Model Obesity with Type 2 Diabetes Program of Nutrition Science , Sebelas Maret University , Surakarta , Central Java , Indonesia 2 Department of Pharmacology , Faculty of Medicine , Sebelas Mar,” Jurnal Ilmu Kesehatan, vol. 17, no. 3, pp. 548–554, 2023.
F. Husna, F. D. Suyatna, W. Arozal, and E. H. Purwaningsih, “Model Hewan Coba pada Penelitian Diabetes Animal Model in Diabetes Research,” Mini Review Article Pharmaceutical Sciences and Research (PSR), vol. 6, no. 3, pp. 131–141, 2019.
R. Salto, D. Gir, C. Ortiz-moral, M. Manzano, and J. M. Lopez-pedrosa, “Dietary Complex and Slow Digestive Carbohydrates,” 2020.
R. Ruze et al., “Obesity and Type 2 Diabetes Mellitus: Connections in Epidemiology, Pathogenesis, and Treatments,” Frontiers in Endocrinology, vol. 21, no. 14, p. 1161521, Apr. 2023, doi: 10.3389/fendo.2023.1161521.
. Firdayani, S. Kusumaningrum, and Y. R. Miranti, “Khasiat Umbi Bawang Dayak (Eleutherine Palmifolia (L.) Merr.) Sebagai Herbal Antimikroba Kulit,” Jurnal Bioteknologi & Biosains Indonesia (JBBI), vol. 4, no. 2, p. 85, 2017, doi: 10.29122/jbbi.v4i2.2589.
E. Kumalasari, S. Yugo, Y. R. Maulida, and D. R. Febrianty, “Pengaruh Pemberian Ekstrak Etanol Daun Ramania (Bouea macrophylla Griffith) Terhadap Penurunan Kadar Gula Darah Mencit Putih (Mus muscullus) Yang Diinduksi Aloksan,” Journal Current Pharmaceutical Sciences, vol. 2, no. 2, pp. 2598–2095, 2019.
S. J. Maleki, J. F. Crespo, and B. Cabanillas, “Anti-inflammatory effects of flavonoids,” Food Chemistry, vol. 299, no. March, 2019, doi: 10.1016/j.foodchem.2019.125124.
A. A. Kurniawan and Y. N. S. Wuryaningsih, “Rekomendasi Latihan Fisik Untuk Diabetes Melitus Tipe 2,” Berkala Ilmiah Kedokteran Duta Wacana, vol. 1, no. 3, p. 197, 2016, doi: 10.21460/bikdw.v1i3.22.
M. S. Burhans, D. K. Hagman, J. N. Kuzma, K. A. Schmidt, and M. Kratz, “Contribution of adipose tissue inflammation to the development of type 2 diabetes mellitus,” Comprehensive Physiology, vol. 9, no. 1, pp. 1–58, 2019, doi: 10.1002/cphy.c170040.
J. Tandi, H. K. Muttaqin, K. R. Handayani, S. Mulyani, and R. Patala, “Uji Potensi Metabolit Sekunder Ekstrak Kulit Buah Petai (Parkia speciosa Hassk) terhadap Kadar Kreatinin dan Ureum Tikus Secara Spektrofotometri UV-Vis,” KOVALEN: Jurnal Riset Kimia, vol. 6, no. 2, pp. 143–151, 2020, doi: 10.22487/kovalen.2020.v6.i2.15225.
A. Febrinda, M. Astawan, T. Wresdiyati, and N. Dewi Yuliana, “Kapasitas Antioksidan Dan Inhibitor Alfa Glukosidase Ekstrak Umbi Bawang Dayak,” Jurnal Teknologi dan Industri Pangan, vol. 24, no. 2, pp. 161–167, 2013, doi: 10.6066/jtip.2013.24.2.161.
H. Supri, “Hubungan HBA1c Terhadap Kadar Glukosa Darah Pada Penderita Diabetes Mellitus Di RSUD. Abdul Wahab Syahranie Samarinda Tahun 2016,” Jurnal Husada Mahakam, vol. IV, no. 3, pp. 171–180, 2016.
A. Febrinda, M. Astawan, T. Wresdiyati, and N. Dewi Yuliana, “Kapasitas Antioksidan Dan Inhibitor Alfa Glukosidase Ekstrak Umbi Bawang Dayak,” Jurnal Teknologi dan Industri Pangan, vol. 24, no. 2, pp. 161–167, 2013, doi: 10.6066/jtip.2013.24.2.161.
Y. Hendrawan, E. H. Herdiningsih, D. M. Maharani, and L. C. Hawa, “Effect of ultrasonic assisted extraction on Dayak onion powder extraction (Eleutherine palmifolia),” IOP Conference Series: Earth and Environmental Science, vol. 475, no. 1, 2020, doi: 10.1088/1755-1315/475/1/012015.
S. Dicky and M. Mukhamad, “Pengelolaan Ganguan Inegritas Kulit Pada Ulkus Diabetikum Di Dusun Tegal Melik Desa Gebugan Kecamatan Bergas Kabupaten Semarang,” Media Informasi Penelitian Kabupaten Semarang, vol. 3, no. 2, pp. 80–88, 2021, doi: 10.55606/sinov.v4i2.38.
C. Y. Sianturi, “Manfaat Lidah Buaya Sebagai Anti Penuaan Melalui Aktivitas Antioksidan,” Essence of Scientific Medical Journal, vol. 17, no. 1, pp. 34–38, 2019.